PerkinElmer 16S and 18S Prep Kits

PerkinElmer 16S and 18S Prep Kits

688.0011,030.00

16S and 18S rRNA Library Preparation Kits for Metagenomics Studies

16S ribosomal RNA (rRNA) sequencing is an amplicon sequencing technique used to identify and compare species of bacteria present within a given sample. 16S rRNA gene sequencing is used to study phylogeny and taxonomy of samples from complex microbiomes or environments that are difficult or impossible to study. 16S rRNA sequencing can be used to specify taxonomic assignments, down to the species level. PerkinElmer offers NEXTFLEX® 16S Amplicon-Seq Kits for this purpose, which are optimized library prep kits developed to simplify 16S rRNA sequencing on the Illumina® MiSeq® sequencing platform. Automation protocols are also now available for the PerkinElmer Sciclone® NGS and NGSx Workstations.
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Image Variation SKU Price Quantity Add To Cart
16S V4 Amplicon Seq Kit 2.0 - 4 Barcodes 8 Rxns NOVA 4203-01 688.00
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16S V4 Amplicon Seq Kit 2.0 - 12 Barcodes 24 Rxns NOVA 4203-02 1,613.00
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16S V4 Amplicon Seq Kit 2.0 - 24 Barcodes 48 Rxns NOVA 4203-03 5,809.00
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16S V4 Amplicon Seq Kit 2.0 - Barcodes (1 96) 192 Rxns NOVA 4203-04 11,030.00
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16S V4 Amplicon Seq Kit 2.0 - Barcodes (97 192) 192 Rxns NOVA 4203-05 11,030.00
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16S V4 Amplicon Seq Kit 2.0 - Barcodes (193 288) 192 Rxns NOVA 4203-06 11,030.00
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16S V4 Amplicon Seq Kit 2.0 - Barcodes (289 384) 192 Rxns NOVA 4203-07 11,030.00
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16S V5 V6 Amplicon Seq Kit - 4 Barcodes 8 Rxns NOVA 4205-01 688.00
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16S V5 V6 Amplicon Seq Kit - 12 Barcodes 24 Rxns NOVA 4205-02 1,613.00
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16S V5 V6 Amplicon Seq Kit - 24 Barcodes 48 Rxns NOVA 4205-03 5,809.00
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16S V5 V6 Amplicon Seq Kit - Barcodes (1 96) 192 Rxns NOVA 4205-04 11,030.00
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16S V5 V6 Amplicon Seq Kit - Barcodes (97 192) 192 Rxns NOVA 4205-05 11,030.00
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16S V5 V6 Amplicon Seq Kit - Barcodes (193 288) 192 Rxns NOVA 4205-06 11,030.00
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16S V5 V6 Amplicon Seq Kit - Barcodes (289 384) 192 Rxns NOVA 4205-07 11,030.00
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16S V1 V3 Amplicon Seq Kit - 4 Barcodes 8 Rxns NOVA 4202-01 688.00
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16S V1 V3 Amplicon Seq Kit - 12 Barcodes 24 Rxns NOVA 4202-02 1,613.00
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16S V1 V3 Amplicon Seq Kit - 24 Barcodes 48 Rxns NOVA 4202-03 5,809.00
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16S V1 V3 Amplicon Seq Kit - Barcodes (1 96) 192 Rxns NOVA 4202-04 11,030.00
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16S V1 V3 Amplicon Seq Kit - Barcodes (97 192) 192 Rxns NOVA 4202-05 11,030.00
Add to cart
16S V1 V3 Amplicon Seq Kit - Barcodes (193 288) 192 Rxns NOVA 4202-06 11,030.00
Add to cart
16S V1 V3 Amplicon Seq Kit - Barcodes (289 384) 192 Rxns NOVA 4202-07 11,030.00
Add to cart
18S ITS Amplicon Seq Kit - 4 Barcodes 8 Rxns NOVA-4210-01 688.00
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18S ITS Amplicon Seq Kit - 12 Barcodes 24 Rxns NOVA-4210-02 1,613.00
Add to cart
18S ITS Amplicon Seq Kit - 24 Barcodes 48 Rxns NOVA-4210-03 5,809.00
Add to cart
18S ITS Amplicon Seq Kit - Barcodes (1 96) 192 Rxns NOVA-4210-04 11,030.00
Add to cart
18S ITS Amplicon Seq Kit - Barcodes (97 192) 192 Rxns NOVA-4210-05 11,030.00
Add to cart
18S ITS Amplicon Seq Kit - Barcodes (193 288) 192 Rxns NOVA-4210-06 11,030.00
Add to cart
18S ITS Amplicon Seq Kit - Barcodes (289 384) 192 Rxns NOVA-4210-07 11,030.00
Add to cart

Description

Culture-free Identification of Bacteria

The NEXTFLEX 16S Amplicon-Seq Kits enable analysis of the entire microbial community within a sample, without bias introduced by the culturing required for other, non-NGS based, 16S rRNA analysis methods. With the ability to multiplex hundreds of samples in a sequencing run, NGS-based 16S rRNA sequencing is a sensitive and cost-effective technique to identify strains that may not be identified using other methods.

NEXTFLEX® 16S V4 Amplicon-Seq Kit 2.0
Validated library prep for 16S V4 rRNA sequencing using Illumina® sequencing primers.

NEXTFLEX® 16S V5-V6 Amplicon-Seq Kit
Validated library prep for bacterial profiling of plant samples using Illumina® sequencing platforms.

NEXTFLEX® 16S V1-V3 Amplicon-Seq Kit
Validated library prep for 16S V1-V3 bacterial metagenomics analysis.

Simplifying 18S rRNA Sequencing for Identification of Fungal and Micro-eukaryotic Species

The NEXTFLEX® 18S ITS Amplicon-Seq Kit is designed to prepare multiplexed amplicon libraries that span the hypervariable Internal Transcribed Spacer (ITS) region of eukaryotic 18S ribosomal RNA (rRNA) genes.

NEXTFLEX® 18S ITS Amplicon-Seq Kit

Validated library prep for 18S ITS metagenomics analysis of eukaryotes.

Additional information

Type

16S V4 Amplicon-Seq Kit 2.0, 16S V5-V6 Amplicon-Seq Kit, 16S V1-V3 Amplicon-Seq Kit, 18S ITS Amplicon-Seq Kit

Unit

4 Barcodes – 8 rxns, 12 Barcodes – 24 rxns, 24 Barcodes – 48 rxns, Barcodes (1-96) 192 rxns, Barcodes (97-192) 192 rxns, Barcodes (193-288) 192 rxns, Barcodes (289-384) 192 rxns

Documents

16S V4 Amplicon-Seq Kit 2.0 User Manual

16S V5-V6 Amplicon-Seq Kit User Manual

 16S V1-V3 Amplicon-Seq Kit User Manual

18S ITS Amplicon-Seq Kit User Manual

Sciclone Automation Guide 

 

 

 

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