PerkinElmer NEXTflex Small RNA-Seq Kit v3

PerkinElmer NEXTflex Small RNA-Seq Kit v3

2,213.00

Severe ligation bias in small RNA sequencing is the primary reason why small RNA sequencing data does not accurately reflect the relative abundance of small RNAs in starting material. The NEXTflex® Small RNA Kits solve this problem by incorporating randomized adapters to reduce ligation bias, offering superior data quality and a greater proportion of productive sequencing reads than any other small RNA library prep protocol. Additionally, the NEXTflex® Small RNA-Seq Kits feature a completely gel-free protocol with normal input amounts and a separate protocol for low-input small RNA library preparation.

SKU: NOVA-5132-05 Category:
NOVA-5132-05

8 rxn

Description

  • Completely gel-free protocol with normal input amounts
  • Greater discovery/detection rates reduces sequencing cost
  • Randomized adapters reduce ligation bias, resulting in more accurate data
  • Low input (1 ng) gel-based protocol included
  • AIR Ligase offers greater sequencing depth
  • Use more of your RNA sample – 10.5 µL input volume
  • Barcoded primers are included in the kit allowing multiplexing of up to 48 samples
  • An automation protocol is available for the PerkinElmer® Sciclone® G3 NGS/NGSx and the Zephyr®G3 NGS workstation
  • Functionally validated with Illumina® sequencing platforms

Documents

User Manual

Sequences of NEXTFLEX Small RNA-Seq Barcode Indexes – Excel / PDF

NEXTflex Small RNA-Seq Verson 3 Automation Guide – Sciclone

NEXTflex Small RNA-Seq Verson 3 Automation Guide –  Zephyr

Price Quote

Reference Description Quantity Price Add to Cart
NOVA-5132-05 NEXTflex Small RNA Sequencing Kit v3 8 rxn 2213