Adapters – TruSeq

Adapters – TruSeq

630.0028,000.00

Cost effective options for use with Illumina’s TruSeq NGS sequencing:

  • Unique Dual Index (UDI) Adapters 
  • Single Index Adapters
  • Dual Index Adapters
  • Unique Molecular Dual Indexed (UMI) Adapter Duplexes

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Image Variation SKU Price Quantity Add To Cart
IDT UDI's 25 Rxn - IDT-UDI Per Adaper 630.00
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PE 96 UDI Kit 2 Rxn - NOVA 514150 10,500.00
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PE 96 Dual Index Set A - NOVA 514160 28,000.00
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PE 96 Dual Index Set B - NOVA 514161 28,000.00
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PE 96 HT Barcode Kit 8 Rxn - NOVA 514170 8,500.00
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TS LT Kit A 600 Pmol - IDT-TS-LT-A-600p 3,956.94
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TS LT Kit A 1.2 Nmol - IDT-TS-LT-A-1.2nm 6,594.88
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TS LT Kit B 600 Pmol - IDT-TS-LT-B-600p 3,956.94
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TS LT Kit B 1.2 Nmol - IDT-TS-LT-B-1.2nm 6,594.88
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IDT UMI's 25 Rxn - IDT-UMI-per Adapter 630.00
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Description

Unique Dual Indexed (UDI) Adapters 

  • Illumina recommends using UDIs as a method to mitigate errors introduced by index-hopping. Suitable for both paired-end and single-end sequencing. Each UDI Adapter carries a unique 8-base barcode, 384 barcodes available.
  • IDT xGen UDI Duplexes: Available as individual Adapters, delivered as a 15 uM solution (2nmole), sufficient for ~25 reactions.
  • PerkinElmer NEXTflex UDI Kit: The kit includes 96 barcodes plus Primer Mix, supplied in 96-well plates, each well sufficient for 2 reactions, enabling 192 total reactions.

PerkinElmer Dual Index Adapter Kits – Best price for large projects 

  • Kit contains 96 combinatorial dual indexed barcoded adapters plus Primer Mix
  • Supplied in a 96-well plates, each well sufficient for 8 reactions, enabling 768 total reactions.
  • Each indexed adapter carries an 8-base barcode on each end to enable unique identification.
  • Two different kits available, pooling both enables up to 192 samples on a single flow cell

Single Index Adapters

  • IDT TS-LT Single Index Adapter Kit A: Includes TS Amplification Primer Mix, plus the 12 indexed Adapters of Illumina Kit A.
  • IDT TS-LT Single Index Adapter Kit B: Includes TS Amplification Primer Mix, plus the 12 indexed Adapters of Illumina Kit B.
    • Each indexed adapter is delivered annealed to the Universal Adapter, delivered at a concentration of 15 µM
    • Two sizes available: 600 pmol (for 8 rxns of 5µl) and 1.2 nmol (for 16 rxns of 5µl)
  • PerkinElmer NEXTflex-HT Barcode Kit: The kit includes 96 unique single-index barcodes plus Primer Mix, supplied in a 96-well plates, each well sufficient for 2 reactions. Enables multiplexing up to 384 samples per flow cell lane.

IDT’s xGen Unique Molecular Indexed (UMI) Adapter Duplexes

  • UMI Adapters are identical to the UDI Adapters but carry a short sequence of random bases, that incorporate a unique barcode onto each molecule within a given sample library. UMIs increase sensitivity of variant detection and are meant to reduce the rate of false-positive variant calls during SNP detection. UMI adapters are available at the same price as UDI adapters!

Additional information

Unit

TS-LT Kit A 600 pmol, TS_LT Kit B 600 pmol, TS-LT Kit A 1.2 Nmol, TS-LT Kit B 1.2 Nmol, PE 96-UDI Kit 2 rxn, IDT UDI's 25 rxn, IDT UMI's 25 rxn, PE 96-HT Barcode Kit 8 rxn, PE 96-Dual-Index-Set A, PE 96-Dual-Index-Set B

Documents

User Manual PerkinElmer UDI Adapter Kit

User Manual PerkinElmer HT Barcode Kit

User Manual PerkinElmer Dual Index Kit

IDT Single Index and UDI Adapters

IDT Dual Index Unique Molecular Adapters

 

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